Quoted from TaylorVA:A video I shot of the LEDs flashing when right flipper activated
» YouTube video
Hey TaylorVA,
Definitely a connector or intermittant component issue. Notice how all the lights don't do it? The first board in the serial connection (WOZLED06), which is the scarecrow, isn't doing it. I couldn't tell exactly where it was starting, but it didn't look like it was happening on WOZLED05, the Haunted Forest. If that's the case, you already know that half of the playfield behind it is good, so check from that point forward only.
Point being, check from the last board that doesn't flicker in the serial connection, and you'll find your problem.
Eric