Quoted from NimblePin:

Just keep things dark, stock the bar with beers from Milwaukee and play music from bands that have splotchy facial hair, clap for solos and have a triangle player in the band.
You also need some sort of art house/avant-garde film looping in the background as well as a photo booth. Hipsters love pictures! []
You also need ample fixie parking too. (Bike hooks from the ceiling would work.)
Maybe try to find a location downtown, next to a Goodwill store, bike shop, record shop, or Vegan eatery for optimal hipster cred.