Yes, and if you have a tone button (that will play a beep when continuity exists so you don't have to look at the display) it will be helpful. I don't seen one on the face.
Power off for safety. You don't need it on for this test.
Power on multimeter and make sure it's set to continuity. Touch the leads of the two probes together and note what continuity looks like on the display.
So on page C-133 you can see that the EOS switches are all using the black wire as common, and it connects to the board at J-601-1 (the first pin of J601 connector). So jam one probe from your multimeter into the top of J601-1 where the wire comes out of the connector top so the probe will contact the metal bit holding the wire into the connector. Then touch the other probe to the black wire on the Left Flipper EOS switch. Make sure you're getting continuity. If so that means that wire segment is good.
Then touch one probe to the black wire connector on the Left Flipper EOS and the other to the Right Flipper EOS. If you're getting continuity, that segment is good.
Then touch one probe to the black wire connector on the Right Flipper EOS and the other to the Upper Right Flipper EOS. If you're getting continuity, that last segment is good.
If any of those segments don't have continuity, that's your problem. Since all three are out, I would expect that the first segment from J601-1 to the first EOS is the one that's out, but that's only a guess.